Germination tests

The germination test is a key method used to evaluate the viability and germination potential of seeds. Its purpose is to estimate the percentage of seeds that can germinate under optimal conditions to assess the quality of the batch. This control is essential to ensure quality and guarantee the traceability and reliability of stored seed lines. The experimental unit of maize at St Martin de Hinx maintains 6,810 lines, including 2,015 tropical varieties, and its mission is the preservation of the genetic heritage of maize lines.

Essential elements of the work

Preparation : a sample is taken from the seed batch.

Equipment and conditions: the test is generally carried out in flat trays with absorbent paper such as damp blotting paper at the bottom. Sufficient humidity and stable temperature are maintained.

Procedure:

1. Preparation of trays with wet blotters.

2. Labeling, identification of trays, and distribution of the batch to be tested into representative samples.

3. Incubation in a controlled space (suitable temperature and no light).

4. Observation and counting of germinated seeds at defined intervals (after 7 days).

5. Determination of the number of germinated seeds (germination).

Calculation and interpretation: the germination percentage is calculated as the ratio: (number of germinated seeds / number of seeds tested) × 100.

Interpretation and use: a low germination rate may indicate poor batch quality; it is also a criterion for re-multiplying the line and ensuring high germinative quality seeds.

In summary, seed germination testing is a methodical procedure that combines representative sampling, controlled germination conditions, repeated observation, and precise calculation of the germination percentage, all guided by standards to ensure seed quality and reliability.